FastQCFastQC Report
Wed 31 May 2023
EGAF00007923706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479079
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT81781.707025354899714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68871.437549965663283No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG25880.5402031815212105No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG19900.4153803443690915No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC17990.37551218066331443No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG17930.37425977761496537No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17450.3642405532281732No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17120.3573523364622536No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC16740.3494204504893765No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG14710.3070474806869013No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG14090.29410598252062814No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13960.2913924425825386No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC13040.2721889291745203No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11650.24317492522110135No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10670.222719008764734No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG10120.21123864748820131No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9910.20685523681897977No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9880.20622903529480524No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9620.20080195541862617No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9030.18848665877652745No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8970.18723425572817845No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8600.17951110359669284No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8370.17471022524468824No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG8100.16907441152711766No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG8060.16823947616155163No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7800.16281239628537256No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7690.16051632403006602No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG7590.15842898561615099No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7350.1534193734227549No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7110.14840976122935884No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7040.1469486243396183No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7030.1467398904982268No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6840.14277394751178824No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6770.1413128106220477No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6680.1394342060495242No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6590.13755560147700066No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5680.11856082191037387No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5400.11271627435141178No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5370.11209007282723725No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5230.10916779904775623No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5090.10624552526827519No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5080.10603679142688366No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5080.10603679142688366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)