FastQCFastQC Report
Sun 21 May 2023
EGAF00007923708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525009
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT90151.7171134209127845No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31720.6041801188170107No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG28180.5367527032869913No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG21130.4024692910026304No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG20100.38285057970434794No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19390.3693270020132988No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC19380.36913652908807276No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19060.36304139548083936No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18930.3605652474529008No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17250.32856579601492547No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16090.3064709366887044No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG15640.29789965505353244No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC14250.27142391844711233No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG13360.2544718281019944No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12100.23047223952351292No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11080.21104400115045646No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG10920.20799643434683976No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10910.20780596142161373No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT10670.2032346112161887No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10350.19713947760895528No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10110.19256812740353024No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9880.1881872501233312No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG9020.17180657855389145No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8980.1710446868529873No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8880.16913995760072684No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8720.16609239079711013No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG8620.1641876615448497No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8420.16037820304032885No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG8360.15923536548897257No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG8030.1529497589565131No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG7980.15199739433038292No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7930.15104502970425268No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7900.15047361092857456No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7790.1483784087510881No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7460.14209280221862863No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7060.13447388520958686No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG6400.12190267214466799No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6340.12075983459331173No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5810.11066476955633141No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5790.11028382370587933No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5780.11009335078065328No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5520.10514105472477614No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5350.1019030149959334No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5350.1019030149959334No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5260.10018875866889901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)