FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007923710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484192
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT82061.6947822351463884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68441.41348886392175No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG26360.5444121340294759No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG19620.40521115590509543No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18290.3777427136342608No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG18240.3767100654285903No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17770.36700317229528784No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17470.3608072830612649No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC17430.35998116449672857No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG15070.31124016918908204No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14810.3058703985195955No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG14340.29616350538629305No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC13260.2738583041438107No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG12460.2573359328530831No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10460.21603000462626396No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA10270.21210594144471612No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG9710.20054028154120682No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9680.1999206926178045No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9560.19744233692419538No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9220.1904203291256361No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9130.18856156235542926No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9070.18732238450862468No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8790.18153955455687001No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7920.1635714757782037No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7870.1625388275725332No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7800.16109312008459456No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7620.15737558654418082No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG7530.15551681977397394No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7500.15489723085057167No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7310.15097316766902386No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7300.15076663802788978No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7060.14580992664067147No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7010.144777278435001No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6980.14415768951159869No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6810.14064668561231908No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6330.1307332628378825No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5820.12020025114004362No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5710.11792842508756857No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5570.11503701011169123No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5450.11255865441808209No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5220.10780847267199789No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5190.1071888837485956No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5130.10594970590179104No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5060.10450399841385236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)