FastQCFastQC Report
Wed 31 May 2023
EGAF00007924327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536141
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA44840.8363471549461802No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA42350.7899041483490351No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC29190.5444463303496655No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28670.5347473892129122No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA20640.38497335588958875No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20160.37602048714797043No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19600.36557547361608234No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16580.30924700778340025No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15280.28499965494151724No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12750.23781057594923724No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12310.22960377960275377No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11600.21636099458910998No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11080.20666205345235678No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA10080.18801024357398521No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9990.18633158068493175No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9720.18129559201777143No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9090.16954495179439735No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8600.16040556495399533No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT8270.1542504676941327No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8170.15238528670629556No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8110.15126617811359327No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8000.14921447902697238No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7820.1458571532488655No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG7740.14436500845859576No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7680.14324589986589348No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC7580.1413807188780563No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT7550.14082116458170518No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7350.13709080260603088No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7310.13634473021089602No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6920.1290705243583311No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6720.1253401623826568No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6690.12478060808630564No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6300.11750640223374074No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT5830.10874005159090612No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5780.10780746109698755No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5570.10389058102252952No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5440.10146584573834122No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5410.10090629144199006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)