FastQCFastQC Report
Wed 31 May 2023
EGAF00007924330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510391
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT96811.896781095277934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49230.9645546257673039No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37190.7286570492034539No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26530.5197975669633673No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21710.4253601650499323No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20160.3949912909906326No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18600.3644264887115956No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA16390.3211263521496265No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15240.29859460687982353No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14490.2838999903995172No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14400.28213663642188047No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12490.24471434645203383No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11050.2165006828098458No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10650.2086635540203491No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9900.19396893754004282No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9670.18946258848608225No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9290.1820173161360604No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9180.1798621057189488No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9170.17966617749921138No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8810.17261276158866437No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8680.17006569473207797No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8510.16673491499654186No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8430.16516748923864252No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8320.16301227882153094No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7750.15184437029649817No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7670.15027694453859886No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7490.14675023658332534No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7390.14479095438595116No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7100.13910903601356606No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6690.13107597900433196No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6620.12970448146617006No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6600.12931262502669522No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6460.12656962995037138No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6410.1255899888516843No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6030.11814471650166244No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5860.11481393676612636No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5830.1142261521069141No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5760.11285465456875218No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5740.11246279812927736No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5650.11069944415164061No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5610.10991573127269094No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5450.10678087975689228No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5280.10345010002135617No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5200.10188267426345685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)