FastQCFastQC Report
Sun 21 May 2023
EGAF00007924331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543140
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA44000.8101042088595942No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA42850.7889310306734911No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC31180.5740693007327761No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28240.5199396104135213No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA21310.39234819751813527No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20500.37743491549140185No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19630.3614169459071326No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16990.31281069337555695No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15350.28261590013624477No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13180.242663033472033No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13100.2411901167286519No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11340.20878594837426814No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11310.20823360459550025No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9950.18319401995802187No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9780.1800640718783371No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA9780.1800640718783371No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9540.17564532164819383No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9010.16588724822329418No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8950.16478256066575836No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8430.15520860183378135No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT8040.1480281327097986No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC7980.14692344515226277No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7970.14673933055934013No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG7860.14471407003719114No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7620.1402953198070479No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7430.13679714254151784No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7330.1349559966122915No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT7180.13219427771845196No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6880.1266708399307729No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6760.12446146481570129No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6710.12354089185108812No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6650.12243620429355231No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6330.116544537320028No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6230.11470339139080163No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6050.1113893287181942No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5940.10936406819604522No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5720.10531354715174723No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5630.10365651581544352No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5470.10071068232868137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)