FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543140
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT97771.8000883750046028No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42370.7800935302132047No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28950.5330117465110285No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22860.42088595942114376No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22450.41333726111131575No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20700.38111720734985455No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA17560.32330522517214716No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17270.3179659019773907No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15970.29403100489744816No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15830.2914534005965313No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14030.258312773870457No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12280.22609272010899584No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10810.1990278749493685No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10570.19460912471922523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10410.1916632912324631No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10270.18908568693154618No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9990.1839304783297124No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9860.18153698862171816No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9570.17619766542696175No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9440.1738041757189675No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9350.1721471443826638No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9220.16975365467466952No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8850.162941414736532No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8480.15612917479839453No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8220.151342195382406No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7970.14673933055934013No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7970.14673933055934013No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7690.14158412195750636No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7620.1402953198070479No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA7220.1329307360901425No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7040.12961667341753508No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7000.12888021504584454No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6980.12851198585999926No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6860.12630261074492766No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6860.12630261074492766No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6810.12538203778031448No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6680.12298854807232021No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6530.12022682917848068No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6210.11433516220495638No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6110.11249401627573001No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5900.10862760982435467No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5890.10844349523143205No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5880.10825938063850941No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5700.10494531796590198No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5650.1040247450012888No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5550.10218359907206245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)