FastQCFastQC Report
Wed 31 May 2023
EGAF00007924334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518004
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT99181.9146570296754466No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44980.8683330630651501No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39080.7544343286924425No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26450.5106138176539177No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21370.41254507687199327No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20140.38880008648581865No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18980.36640643701593034No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA16030.3094570698295766No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15010.28976610219226107No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA14840.2864842742527085No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14230.27470830341078445No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12530.24189002401525855No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11180.21582844920116448No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10000.19304870232662297No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9930.1916973614103366No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9790.1889946795777639No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9670.1866780951498444No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9370.1808866340800457No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8990.17355078339163404No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8980.17335773468930743No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8560.16524968919158925No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8430.16274005606134315No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8160.15752774109852433No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7990.15424591315897174No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7990.15424591315897174No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7720.14903359819615292No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7490.1445934780426406No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7410.14304908842402764No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7390.14266299101937438No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA7220.13938116307982176No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6610.12760519223789776No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6600.12741214353557115No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6530.1260608026192848No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6260.12084848765646597No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6120.11814580582389325No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6010.1160222700983004No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6010.1160222700983004No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5720.11042385773082834No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5650.10907251681454197No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5440.1050184940656829No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5360.1034741044470699No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5300.10231581223311018No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5280.10192971482845692No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5230.1009644713168238No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5230.1009644713168238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)