FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482692
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA71671.4847977592336314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47470.983442857971543No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA36870.7638411243608761No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34260.7097693767454194No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA31840.6596338866192106No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22160.4590919261143752No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19760.40937077888177137No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18440.3820241479038393No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17030.3528129739046845No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16510.3420400586709537No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14950.30972131296976124No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC14350.2972910261616103No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13300.27553802424734614No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC13270.2749165099069386No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13170.27284479543891343No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12660.2622790516519851No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12560.26020733718396No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12360.25606390824790964No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12100.25067745063104424No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9750.201992160632453No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG9670.20033478905803287No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8850.18334673042022656No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8580.17775310135655864No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8550.1771315870161511No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8480.17568138688853346No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8450.1750598725481259No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8090.16760170046323533No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8080.16739452901643284No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7950.1647013002080001No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7890.16345827152718503No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7280.15082081327223157No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6560.13590446910245044No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6320.13093235437919004No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6310.13072518293238752No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5870.12160963927307683No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5840.12098812493266929No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5690.11788055323063154No Hit
ATCCACGGTTCTCCGGGCACTGGTAAATCCC5610.1162231816562114No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5530.1145658100817913No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5320.11021520969893846No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5170.10710763799690072No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG5100.1056574378692831No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5070.10503592352887556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)