FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489768
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC35010.714828245209977No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT32830.6703173747570278No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC25690.5245340651083779No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC24930.5090165139412947No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23290.4755312719491677No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19750.4032521520393329No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG17780.3630290259878146No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16230.3313813887391581No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA16200.330768853824668No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC16110.3289312490811976No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG15850.3236226131556165No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15220.3107593799513239No Hit
ACGGCGGAAGGCATACGGGTTGTCTTCGTCTGGCGCATCAATAT14680.29973375149050163No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13140.26829029254667514No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12860.262573300011434No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT12770.2607356952679636No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT12720.2597148037438134No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG12450.2542019895134022No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11780.24052204308978947No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11570.23623429868835857No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9850.20111563025759135No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9610.19621535094167036No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9450.19294849806438968No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC9230.18845657535812874No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8650.17661423367798632No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8380.17110141944757518No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7760.15844236454811259No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA7580.1547671550611718No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7470.15252119370804135No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7400.15109194557423108No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6810.1390454255892586No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6780.13843289067476844No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6350.12965322356707665No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC6000.1225069828980252No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5920.12087355645938486No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5840.11924013002074452No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA5690.11617745544829389No Hit
CGGGTTCTGCATGATATCATCCATGATCACTACGCTCTGCTGGT5410.11046046291305271No Hit
GTTGCGTGGGCCGGTCGGCAGGGTATCTGCTACACGAGCTACCG5400.11025628460822268No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA5220.10658107512128191No Hit
CTCACCTACAGAAACTTTAGTGTTGTTGCCTTTGTCACCGAACT5190.10596854020679179No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5130.10474347037781152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)