FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489768
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA74771.5266411852142239No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45020.919210728344849No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA36660.7485176655069339No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34930.7131948187713366No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA31430.641732412080822No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22800.46552653501249575No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20300.4144819588049852No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18310.37385047614380684No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17980.3671125920844155No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17150.350165792783522No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15580.3181097989252054No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC14380.29360840234560037No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13980.2854412701523987No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13910.2840120220185884No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC13650.27870338609300727No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13010.2656359745838846No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12420.25358945459891213No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12360.2523643847699319No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12330.25175184985544174No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9870.20152398686725143No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9700.19805295568514075No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG9500.19396938958853988No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9130.18641479230982833No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9100.1858022573953382No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8920.18212704790839743No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8850.18069779977458716No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8740.1784518384214567No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8140.16620114013165418No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8000.16334264386403358No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7570.15456297675634179No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7400.15109194557423108No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7180.14660002286797014No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7090.14476241812449975No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6280.12822397543326639No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5980.12209862628836511No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5930.12107773476421489No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5850.11944430832557455No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5540.11311478087584326No Hit
ATCCACGGTTCTCCGGGCACTGGTAAATCCC5530.11291060257101321No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC5450.11127717613237287No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5430.1108688195227128No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG5420.11066464121788275No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5320.10862285816958234No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA5070.1035184005488313No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4960.10127243919570084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)