FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491301
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA49621.009971483876483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41730.8493774692093035No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23240.47302977197278245No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA18520.3769583208664342No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18410.37471936755675234No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC17540.3570112822892687No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15180.30897555673609456No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC13370.2721345977313297No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11640.2369219684063334No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11120.2263378254878374No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9100.18522250107367988No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA9040.18400125381385343No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8970.1825764653440559No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8260.16812503943610943No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8090.1646648388666011No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7330.1491957069087993No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7020.1428859293996959No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6450.13128408043134454No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6070.12354951445244361No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6040.12293889082253039No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6020.12253180840258823No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA6020.12253180840258823No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5940.12090347872281962No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5610.11418661879377408No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5590.11377953637383192No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5360.10909808854449715No Hit
CTGGACAGCCTGCGCATGGAGATGGAACGTC5260.1070626764447864No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC5180.10543434676501778No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5130.10441664071516238No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5100.10380601708524916No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA5000.10177060498553839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)