FastQCFastQC Report
Sun 21 May 2023
EGAF00007924360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507191
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52571.0364931554384837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50490.9954829640115854No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33630.6630638161954767No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22080.43533895514707477No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21400.4219317771805888No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA19270.37993576384439No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18740.3694860516058053No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18650.3677115721690645No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17770.3503611065653767No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13980.2756358058404033No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13300.2622286278739173No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12730.2509902581078923No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12160.23975188834186728No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12030.23718875137768614No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11420.22516172408422072No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11160.22003545015585846No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10480.20662827218937246No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9470.18671466962150354No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9180.18099690254756098No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9140.18020824502012062No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA8240.1624634506527127No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC7940.15654851919691004No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7920.15615419043318987No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7870.1551683685238894No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7850.15477403976016924No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7820.154182546614589No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC7720.1522109027959881No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7700.15181657403226792No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7530.1484647795406464No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7510.14807045077692624No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG7510.14807045077692624No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7280.14353566999414422No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT7230.14254984808484378No Hit
GAAGATGGCAACAAACCGGGTAAGGAAGACG7050.1390008892113622No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7040.1388037248295021No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6890.13584625910160078No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6880.13564909471974068No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6520.12855117697277751No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG6340.12500221809929593No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG6300.12421356057185558No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6200.12224191675325469No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5950.11731280720675248No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5820.11474967024257134No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5700.11238369766025029No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5610.11060921822350948No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5550.10942623193234896No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5510.10863757440490861No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5150.10153965665794544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)