FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537742
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT54951.0218655042752844No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37410.6956867791617541No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24880.4626754093970714No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22580.41990396881776015No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21560.4009357647347613No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20680.3845710396435465No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19360.36002395200672443No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18260.339568045642706No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15480.28787039137727755No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14550.27057585236042564No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13310.24751646700462304No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13220.24584280193847607No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13110.2437972113020742No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12930.24044988116978028No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11640.2164606818883405No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11430.21255546340066428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10870.20214154743352758No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9400.17480501801979387No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9250.17201557624288227No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9120.16959806003622555No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8810.1638332136972749No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8470.15751047900294193No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG8460.1573245162178145No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8450.15713855343268704No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8350.15527892558141265No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8250.15341929773013824No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8140.1513737070937364No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG8090.1504438931680992No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8050.14970004202758944No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8030.14932811645733457No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8010.14895619088707968No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7860.14616674911016805No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC7830.14560886075478574No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7830.14560886075478574No Hit
GAAGATGGCAACAAACCGGGTAAGGAAGACG7750.14412115847376622No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7560.14058786555634487No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7300.1357528331430314No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7070.1314756890851003No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6940.12905817287844357No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6620.12310736375436548No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG6510.12106177311796364No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6490.12068984754770874No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6330.11771444298566971No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6140.11418115006824835No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5900.10971804322518978No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5850.10878822929955258No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5750.10692860144827818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)