FastQCFastQC Report
Wed 31 May 2023
EGAF00007924364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512215
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52721.029255293187431No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47420.925783118417071No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35530.6936540319982819No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22270.4347783645539471No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21080.41154593286022473No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19730.3851898128715481No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA19620.38304227716876704No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17810.34770555333209685No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17690.3453627871108812No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14820.28933162832013903No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13830.2700038069951095No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12300.24013353767460927No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12290.23993830715617467No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12100.23622892730591646No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11660.22763878449479225No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10990.21455833975967126No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10050.1962066710268149No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9080.17726931073865465No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8930.17434085296213503No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8440.1647745575588376No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8290.16184609978231798No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA8290.16184609978231798No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8200.1600890251164062No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7950.15520826215554015No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7920.15462257060023624No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC7730.15091319074997803No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG7730.15091319074997803No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7700.1503274991946741No Hit
GAAGATGGCAACAAACCGGGTAAGGAAGACG7610.14857042452876235No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7420.14486104467850414No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC7330.14310397001259237No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7280.14212781742041916No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7270.14193258690198451No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT7210.14076120379137666No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG7050.1376375154964224No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6970.13607567134894527No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6610.12904737268529817No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6460.12611891490877852No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6450.1259236843903439No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6280.12260476557695499No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6110.11928584676356609No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5980.11674785002391574No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5760.11245277861835362No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5740.11206231758148434No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5690.11108616498931112No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5610.109524320841834No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5330.10405786632566402No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5270.10288648321505618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)