FastQCFastQC Report
Sun 21 May 2023
EGAF00007924366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509931
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG39330.7712808203462821No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA37670.7387273964516768No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23540.46163108342109027No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21510.42182177588732594No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC19180.37612931945694617No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG18140.35573440328201267No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA17330.339849901261151No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT17160.33651611688640226No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC15940.31259131137349955No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG15380.3016094334331508No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14390.2821950420743199No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13530.26533001523735567No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13070.256309186929212No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA12980.25454424226022737No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11690.22924670200478106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10110.19826211781593983No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10110.19826211781593983No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10020.1964971731469552No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9910.19434001855152952No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9900.19414391358830901No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG9140.17923993638354993No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9110.17865162149388839No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG8870.17394510237659605No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8650.16963079318574473No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8380.16433595917879087No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8100.15884502020861646No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC7930.15551123583386772No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7900.15492292094420618No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG7850.15394239612810362No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7770.1523735564223395No Hit
AAAGCCATTATCAACCTGGCAGGCTCTTCCT7770.1523735564223395No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7710.1511969266430164No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7550.1480592472314882No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG7200.14119557351877018No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7140.1400189437394471No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6650.13040980054164192No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6580.12903706579909832No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6190.12138897223349825No Hit
AGCGGTTTCCGTAAAACCAACATGTCTCTGG6180.12119286727027775No Hit
CCACCGCGTACCCCGACTTGGGAATCTCCGG6060.11883960771163157No Hit
CCGCCTCGTACCCCAACCTGGGAATCTCCGG6010.117859082895529No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5940.1164863481529854No Hit
TCTGAAAACACCCAACAGACCAGCACGAAAC5910.11589803326332386No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5860.1149175084472213No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5600.10981877940348793No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5410.10609278510229816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)