FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462629
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC40760.8810515553499673No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT37400.8084231641336795No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG30350.6560332361352185No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23500.5079664266615366No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23270.5029948403580408No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22780.49240319997233206No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18990.4104801039277693No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17890.38670295204148464No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14700.31774921157125907No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG13500.29181050042258483No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12530.2708433755774065No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11510.24879547110103345No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11380.24598544405992706No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11270.24360772887129858No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11270.24360772887129858No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10940.23647458330541318No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA9630.20815815696811052No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9570.2068612214106768No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9370.2025381028858978No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9250.19994423177103035No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9200.19886345213983558No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8980.19410802176257866No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC8630.18654256434421534No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8530.1843810050818258No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC7420.1603876972693022No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7160.15476764318708944No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6900.14914758910487672No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6720.14525678243257556No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6510.14071750798155758No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6450.13942057242412387No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6350.13725901316173433No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6280.13574592167806168No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6210.13423283019438903No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA6190.13380051834191112No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6000.12969355574337105No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5620.12147963054629086No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5550.11996653906261821No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5480.11845344757894555No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5080.10980721052938748No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5040.10894258682443167No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4870.1052679360783695No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG4830.10440331237341369No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4800.10375484459469683No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG4780.10332253274221892No Hit
ATGGACGTGTCCCAGGATGCAAAAGACTGGC4650.10051250570111256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)