FastQCFastQC Report
Sun 21 May 2023
EGAF00007924384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437164
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41500.9493004913487844No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38170.8731277049345325No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT33890.7752239434171158No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG28090.6425506217346351No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21650.4952374852458116No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG21460.4908912902251787No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20250.46321289035693697No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17700.4048823782379153No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17010.3890988278998271No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13490.3085798464649422No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG13120.3001162035300253No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10950.25047808145226963No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10850.24819061038877857No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10560.24155694430465455No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10500.2401844616665599No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10470.2394982203475126No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10320.236067013752276No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10090.23080583030624663No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9020.20632988992689244No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8520.1948925346094372No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8410.19237631643959704No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8100.1852851561427748No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC8050.18414142061102926No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7770.17773650163325433No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC7510.17178907686817763No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6650.15211682572215462No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6540.14960060755231447No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6200.1418232059364449No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6130.1402219761920012No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6020.13770575802216103No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5860.13404580432057536No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5840.13358831010787714No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5680.12992835640629147No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5630.12878462087454592No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5590.12786963244914953No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA5450.12466717296026204No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5410.12375218453486564No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4840.11071359947296666No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4760.10888362262217383No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG4740.10842612840947562No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG4740.10842612840947562No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4510.10316494496344622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)