FastQCFastQC Report
Sun 21 May 2023
EGAF00007924386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444005
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39950.8997646422900643No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39310.8853503901983086No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT35540.8004414364703101No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG28730.6470647853064717No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG21560.48558011734102097No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21450.4831026677627504No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20680.4657605207148568No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17440.3927883695003434No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16510.3718426594295109No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13890.31283431492888597No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG13020.2932399409916555No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11270.25382597042826094No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11060.2490962939606536No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10820.2436909494262452No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10650.23986216371437258No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10580.2382856048918368No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10500.23648382338036733No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9290.20923187801939166No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8810.19842118895057487No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8650.19481762592763596No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8590.19346628979403385No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8520.19188973097149806No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8120.18288082341415074No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC8040.18107904190268126No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC7890.177700701568676No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7150.1610342225875835No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6840.15405231923063928No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6710.1511244242745014No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6530.1470704158736951No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6070.1367101721827457No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5740.12927782344793415No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5660.12747604193646467No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5630.12680037386966364No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5610.12634992849179627No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA5600.12612470580286259No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5520.12432292429139312No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5500.12387247891352575No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5410.1218454747131226No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG4930.1110347856443058No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4880.10990867219963739No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG4690.1056294411098974No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4670.10517899573203006No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4630.1042781049762953No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC4540.10225110077589215No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4500.10135021002015744No Hit
ATGGACGTGTCCCAGGATGCAAAAGACTGGC4500.10135021002015744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)