FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007924390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427043
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC49201.1521088040314442No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA44991.0535238840116803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41250.9659448814288022No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT38000.8898401331950178No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG32820.7685408729331706No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27510.6441974227419721No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25250.5912753516624789No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24850.5819086134183209No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23740.5559159147907822No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21310.49901297995752186No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20540.48098200883751757No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18400.4308699592312718No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA18370.43016745386295996No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18100.42384490554815324No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16970.39738387000840664No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13460.31519074191591945No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12210.28591968490292546No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11880.27819212585149505No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11520.2697620614317528No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11500.26929372451954486No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11310.26484452385356977No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10770.2521994272239564No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG10230.23955433059434295No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8960.20981493666914103No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8760.20513156754706202No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8710.20396072526654227No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8480.19857485077615136No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG8460.19810651386394343No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7380.17281632060471663No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6460.15127282264315303No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6340.1484628011699056No Hit
GGTACCGGCTACGAAAAACGCGATGACTGGC6340.1484628011699056No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6060.14190608439899496No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTA5740.13441269380366846No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5710.13371018843535665No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5670.13277351461094083No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5400.12645096629613412No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT5210.12200176563015902No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5160.12083092334963927No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4860.1138058696665207No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4710.11029334282496141No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT4690.10982500591275351No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4620.10818582672002584No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG4370.10233161531742706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)