FastQCFastQC Report
Wed 31 May 2023
EGAF00007924393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444661
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT49651.1165809459340936No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT40530.9114808809407616No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT40500.9108062096743361No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT29810.6703983484047398No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG29030.6528568954776784No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG24500.5509815342474379No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23840.5361387663860784No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT22650.5093768061512028No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC22150.4981322850441123No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT22030.4954335999784105No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG21720.48846199689201436No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19750.44415858373007755No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG19380.43583763811083054No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT17760.3994053897238571No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16110.3622984700704582No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14140.3179950569085213No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT13990.31462170057639416No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT13720.30854965917856525No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG13640.30675053580143075No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG13620.30630075495714715No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT13390.30112827524788544No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT13380.30090338482574364No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT12850.2889841924522277No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12670.284936164853675No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12380.2784143426115625No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC12260.27571565754586075No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG11780.26492091728305384No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG11500.25862398546308313No Hit
GTCGGTGCAGTTCAGACAAGTTGGGCACAGTTTACCACGAGTTT11420.2568248620859486No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC10940.24603012182314166No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10850.24400610802386538No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC10420.2343358198717675No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9750.2192681615882661No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA9160.20599962668189925No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8970.20172670866120485No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG8960.20150181823906302No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8680.19520488641909228No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8530.19183153008696513No Hit
CTCACCTACAGAAACTTTAGTGTTGTTGCCTTTGTCACCGAACT8310.18688394079984527No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT8260.18575948868913622No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG8230.18508481742271077No Hit
TTCCTGACCTTCCTCACCTTCGTCGCCATCGCCACCTTCATCAC8030.18058700897987454No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7420.16686869322922404No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7280.16372022731923871No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7160.16102154225353696No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7010.15764818592140978No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6990.15719840507712618No Hit
CATGCATTTCGGACGACCCAGTGCCACGTCCAGATCGGTACAGT6990.15719840507712618No Hit
CATGCATTTTGGGCGGCCCAGGGCAACGTCCAGATCAGTGCAGT6700.15067658283501364No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6490.14595388397003559No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6300.14168096594934118No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6260.14078140426077396No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6180.13898228088363945No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6160.13853250003935583No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6150.138307609617214No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG6080.13673337666222132No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG5960.1340346915965196No Hit
TTCCTGGCCCTCTTCGCCTTCATCACCATCGCCACCTTCGTCGC5940.13358491075223597No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5720.1286373214651161No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5620.126388417243698No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5440.12234038964514539No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT5440.12234038964514539No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5410.12166571837871998No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5380.12099104711229454No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG5360.1205412662680109No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5340.12009148542372729No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5330.11986659500158549No Hit
CTCCTGGCCTTCTTCACCTTCATCGCCGTCACCGCCTTCGTCAC5280.11874214289087641No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5260.1182923620465928No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5050.11356966318161477No Hit
TGGACGCACTTCGTGCAGGATGGACGCTTTTGCGCTCGGGGTAG5050.11356966318161477No Hit
TTCTTGACCTTCTTCGCCCTCATCACCATCGCCGCCTTCATCGC4890.10997141642734579No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4880.10974652600520397No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA4810.10817229305021128No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG4650.1045740462959423No Hit
GTAAGCGCCCTGGGTGGTCTGCATGATAGCACCCGCTTTCGGGG4640.1043491558738005No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4610.10367448460737506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)