FastQCFastQC Report
Sun 21 May 2023
EGAF00007924394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444661
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT47901.0772251220592766No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT42310.9515113760820041No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT40700.9153040181171724No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34770.7819439977870781No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC31570.7099790627016986No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA30790.6924376097746373No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC26390.5934858240322403No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA26160.5883133443229787No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA23310.5242195740125624No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC22450.5048789977083666No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT21540.48441396929346175No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG21240.4776672566292074No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20520.461475146234997No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA17590.3955822525474463No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG16720.3760167858211087No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16450.36994474442327974No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16010.3600495658490401No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA15170.3411587703891279No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14830.3335124960363063No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG14090.31687060479781226No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG14070.31642082395352866No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14050.31597104310924506No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13830.31102345382212515No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT13630.30652564537928895No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13620.30630075495714715No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT13350.30022871355931824No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13110.2948313434279147No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG12690.28538594569795867No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT12220.27481609585729355No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG12130.27279208205801725No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11820.2658204789716211No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG11650.2619973417952103No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT10940.24603012182314166No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10110.22736421678537133No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG9570.2152201339897135No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9420.21184677765758633No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9310.2093729830140264No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG9230.20757385963689193No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9170.20622451710404105No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA8940.20105203739477936No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8820.19835335232907766No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8570.19273109177553238No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT8430.189582625865547No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC8380.18845817375483795No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8290.18643415995556165No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8100.18216124193486724No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7580.17046693998349305No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7430.1670935836513659No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7360.1655193506963732No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7150.16079665183139516No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA7100.15967219972068608No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA7090.15944730929854428No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6640.14932724030216277No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6630.14910234988002097No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA6610.14865256903573734No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6570.1477530073471701No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6400.14392987017075928No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6250.14055651383863213No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6170.13875739046149763No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT6070.13650848624007952No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5950.1338098011743778No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5920.13313512990795234No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5760.12953688315368336No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC5650.12706308851012346No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5650.12706308851012346No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG5610.1261635268215562No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5550.12481418428870533No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5500.12368973217799628No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5360.1205412662680109No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG5280.11874214289087641No Hit
GCAATCAAAGACCTGGTGATGACCAAACCGG5220.11739280035802556No Hit
GCTATCAAAGATCTGGTTATGACCAAACCGG5200.11694301951374192No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5190.11671812909160012No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5130.11536878655874926No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA5090.114469224870182No Hit
CCGGAAGCACCGATCCTGTTCCCGGATGACT4940.11109586853805484No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4910.1104211972716294No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC4870.10952163558306215No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4740.1065980600952186No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4710.10592338882879317No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4660.10479893671808412No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4660.10479893671808412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)