FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420433
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT46001.094110119805058No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT38420.9138198000632681No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT37680.8962188981359694No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT28820.6854837750604734No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG27370.6509955212840096No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG23010.5472929099285736No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22510.5354004086263447No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT21870.5201780069594918No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG20960.49853365458943516No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT20910.4973444044592123No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC20810.4949659041987665No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG17360.4129076452133872No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17230.4098155948748077No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT16210.3855548922182607No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT15490.3684296903430511No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13370.3180054848216006No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG13140.3125349342225753No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT13130.3122970841965307No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG13020.30968073391004036No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT12500.2973125325557223No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT12480.29683683250363313No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12430.29564758237341027No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT12100.2877985315139392No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT11770.2799494806544681No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC11540.2744789300554428No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11380.2706733296387296No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG11260.26781912932619467No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG11210.26662987919597175No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC10280.24450982677382604No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10260.24403412672173688No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC10230.24332057664360313No Hit
GTCGGTGCAGTTCAGACAAGTTGGGCACAGTTTACCACGAGTTT10050.23903927617480075No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8870.21097297310154056No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8670.206215972580649No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG8580.20407532234624778No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA8380.19931832182535622No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8150.19384777122633093No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7710.18338237008036953No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG7690.18290667002828037No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT7670.1824309699761912No Hit
TTCCTGACCTTCCTCACCTTCGTCGCCATCGCCACCTTCATCAC7580.18029031974179002No Hit
CTCACCTACAGAAACTTTAGTGTTGTTGCCTTTGTCACCGAACT7350.17481976914276473No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7150.17006276862187317No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7010.1667328682572491No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6830.16245156778844666No Hit
CATGCATTTCGGACGACCCAGTGCCACGTCCAGATCGGTACAGT6750.16054876758009007No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6670.15864596737173342No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6570.15626746711128764No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG6560.15602961708524307No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6420.152699716720619No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6360.15127261656435154No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6190.1472291661215937No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5900.14033151536630095No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5870.1396179652881672No Hit
CATGCATTTTGGGCGGCCCAGGGCAACGTCCAGATCAGTGCAGT5850.13914226523607803No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5650.1343852647151865No Hit
TTCCTGGCCCTCTTCGCCTTCATCACCATCGCCACCTTCGTCGC5560.13224461448078528No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5330.12677406388176No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5300.12606051380362626No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5170.12296846346504675No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5160.12273061343900217No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5130.12201706336086844No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5090.12106566325669013No Hit
CTCCTGGCCTTCTTCACCTTCATCGCCGTCACCGCCTTCGTCAC5040.11987641312646724No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4900.11654651276184315No Hit
TTCTTGACCTTCTTCGCCCTCATCACCATCGCCGCCTTCATCGC4890.11630866273579857No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT4870.11583296268370942No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT4820.11464371255348652No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG4690.111551662214907No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG4680.11131381218886242No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG4620.10988671203259497No Hit
TGGACGCACTTCGTGCAGGATGGACGCTTTTGCGCTCGGGGTAG4610.1096488620065504No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4590.10917316195446124No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4530.10774606179819377No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA4470.10631896164192631No Hit
AACTTCGTGCAGGATAGAGGCCTTAGCGGACGGAATGGTACCCA4420.1051297115117034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)