FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420433
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT43571.0363125634762258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40260.9575842048554705No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT38200.9085870994902875No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT37760.898121698344326No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31720.7544602826134009No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA29650.7052253272221733No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC28940.6883379753730083No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC24980.5941493650593554No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA23500.558947561204758No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA21560.5128046561521099No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT21110.5021014049801038No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC20360.4842626530267605No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG18950.45072579935447504No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17990.42789219685419555No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15810.3760408911764776No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA15680.3729488408378981No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15520.3691432404211848No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14830.35273158862410897No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13730.3265680857592054No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA13390.3184811848736897No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13240.3149134344830211No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG13140.3125349342225753No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG12760.30349663323288134No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12480.29683683250363313No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT12220.2906527318264741No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT12220.2906527318264741No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12210.29041488180042957No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12110.2880363815399838No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG12020.28589573130558255No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT11240.2673434292741055No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11120.26448922896157057No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG10940.2602079284927682No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG10770.25616447805001036No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT10460.24879112724262845No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9190.21858417393496704No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG8920.21216222323176345No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8870.21097297310154056No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8830.21002157299736224No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8600.20455102239833695No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG8350.19860477174722252No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA8320.19789122166908876No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8200.19503702135655385No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT7810.18576087034081532No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7570.18005246971574543No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7560.17981461968970086No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7240.17220341885627435No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC7120.16934921854373944No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7090.1686356684656057No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7060.16792211838747195No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6980.16601931817911533No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6820.1622137177624021No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6390.15198616664248527No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA6380.15174831661644067No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA6270.1491319663299503No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6150.14627776601741538No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6060.1441371157830142No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5940.14128291547047925No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5750.13676376497563225No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA5700.1355745148454094No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT5690.1353366648193648No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5450.12962826419429493No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5420.1289147141161612No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5410.1286768640901166No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5250.12487126367340337No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5210.12391986356922505No Hit
GCAATCAAAGACCTGGTGATGACCAAACCGG5050.12011426315251181No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG5010.1191628630483335No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC4980.11844931297019977No Hit
GCTATCAAAGATCTGGTTATGACCAAACCGG4970.1182114629441552No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC4820.11464371255348652No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4820.11464371255348652No Hit
CCGGAAGCACCGATCCTGTTCCCGGATGACT4770.11345446242326364No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4710.11202736226699618No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4570.10869746190237209No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG4560.1084596118763275No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4450.10584326158983713No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC4420.1051297115117034No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA4400.10465401145961425No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4360.10370261135543594No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4260.10132411109499016No Hit
CGCCAGCACGGCATCGATCCGTATTCCGTGG4260.10132411109499016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)