FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425496
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT50421.1849700114689679No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT38770.9111719029086055No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT38470.9041213078383816No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT29410.691193336717619No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG27420.6444243894184669No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22950.5393705228721304No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG22780.5353751856656702No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT21590.5074078252204486No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT21420.5034124880139884No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG20960.49260157557297835No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC20670.48578600033842856No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG18300.4300862992836596No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17480.41081467275838085No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT17300.4065843157162465No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16200.3807321337920921No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG13710.32221219470923346No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13670.32127211536653694No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT13420.31539661947468367No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT13250.3114012822682234No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12980.3050557467050219No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT12720.2989452309774945No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT12650.2973000921277756No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG12570.29541993344238254No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT12010.2822588226446312No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11850.2784985052738451No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC11790.27708838625980037No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG10840.2547615018707579No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG10720.2519412638426683No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10630.24982608532160114No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC10510.24700584729351158No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC10360.2434805497583996No Hit
GTCGGTGCAGTTCAGACAAGTTGGGCACAGTTTACCACGAGTTT10170.2390151728805911No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG9070.21316299095643673No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8960.2105777727640213No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8940.21010773309267303No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA8560.20117697933705603No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8330.19577152311655105No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8330.19577152311655105No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT7950.18684076936093405No Hit
TTCCTGACCTTCCTCACCTTCGTCGCCATCGCCACCTTCATCAC7450.17508977757722752No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG7370.17320961889183448No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7350.17273957922048622No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7280.1710944403707673No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7280.1710944403707673No Hit
CTCACCTACAGAAACTTTAGTGTTGTTGCCTTTGTCACCGAACT6990.1642788651362175No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6870.16145862710812792No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6680.15699325023031946No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6420.15088273450279205No Hit
CATGCATTTCGGACGACCCAGTGCCACGTCCAGATCGGTACAGT6380.1499426551600955No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG6270.14735743696768008No Hit
CATGCATTTTGGGCGGCCCAGGGCAACGTCCAGATCAGTGCAGT6250.1468873972963318No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6220.14618233778930942No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6170.14500723861093878No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5890.13842668321206308No Hit
TTCCTGGCCCTCTTCGCCTTCATCACCATCGCCACCTTCGTCGC5720.13443134600560286No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5670.13325624682723222No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5650.13278620715588396No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5640.13255118732020982No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5320.12503055257863765No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5280.1240904732359411No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5250.12338541372891872No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5190.12197529471487394No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5180.1217402748791998No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5140.12080019553650327No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5110.1200951360294809No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5100.11986011619380676No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT5080.1193900765224585No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG5040.11844999717976197No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5000.11750991783706544No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA4960.11656983849436893No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT4760.1118694417808863No Hit
TGGACGCACTTCGTGCAGGATGGACGCTTTTGCGCTCGGGGTAG4650.10928422358847086No Hit
CTCCTGGCCTTCTTCACCTTCATCGCCGTCACCGCCTTCGTCAC4620.10857916408144848No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4590.10787410457442609No Hit
CTGGATGGACGGACGCGGGCGTTTGCTAGGACCCTCGACGTAGT4580.10763908473875194No Hit
TTCTTGACCTTCTTCGCCCTCATCACCATCGCCGCCTTCATCGC4550.10693402523172955No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG4510.10599394588903305No Hit
GTAAGCGCCCTGGGTGGTCTGCATGATAGCACCCGCTTTCGGGG4330.10176358884689868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)