FastQCFastQC Report
Sun 21 May 2023
EGAF00007924398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425496
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT47111.1071784458608307No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT40290.9468949179310734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38010.8933103953973716No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT37810.888609998683889No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33510.7875514693440127No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA30450.7156353996277286No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC29170.6855528606614398No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC24800.5828491924718446No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA23820.5598172485757797No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA22570.5304397691165135No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT20510.4820256829676425No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC20380.47897042510387877No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG19640.46157895726399306No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18290.4298512794479854No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA16880.39671348261793293No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16340.3840224114915299No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15780.37086130069377854No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15060.3539398725252411No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA14480.34030872205614154No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14300.3360783650140072No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG13880.3262075319156937No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG13070.3071709252260891No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12900.30317558801962885No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12770.30012033015586514No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12690.2982401714704721No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT12590.2958899731137308No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12490.2935397747569895No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT12400.2914245962359223No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG12300.289074397879181No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG11180.26275217628367836No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG10910.2564066407204768No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10880.25570158121345443No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT10790.25358640269238725No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT10370.24371556959407373No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG9380.22044860586233478No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9210.21645326865587455No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9170.215513189313178No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG8860.20822757440727996No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8850.20799255457160584No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8780.20634741572188695No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA8150.19154116607441668No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8020.18848590821065297No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7990.1877808487036306No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7950.18684076936093405No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT7690.18073025363340667No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7480.17579483708424992No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7450.17508977757722752No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7170.16850922217835185No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6890.1619286667794762No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC6690.15722827006599355No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA6630.1558181510519488No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6550.15393799236655575No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6500.1527628931881851No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6380.1499426551600955No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA6360.14947261548874724No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA6100.14336209976121983No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6070.14265704025419745No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT5840.13725158403369245No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5810.13654652452667004No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5800.13631150469099593No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5670.13325624682723222No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5440.12785079060672722No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC5360.12597063192133415No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5350.12573561208566003No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG5250.12338541372891872No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5120.12033015586515501No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5050.1186850170154361No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5020.1179799575084137No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4960.11656983849436893No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT4940.11609979882302067No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG4850.11398462030195348No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4830.11351458063060522No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4780.11233948145223456No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4770.11210446161656043No Hit
GCTATCAAAGATCTGGTTATGACCAAACCGG4760.1118694417808863No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC4750.11163442194521218No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA4720.1109293624381898No Hit
GCAATCAAAGACCTGGTGATGACCAAACCGG4710.11069434260251565No Hit
CCGATTCACGAACCGGAGTCCCACAACAGCC4620.10857916408144848No Hit
CCGGAAGCACCGATCCTGTTCCCGGATGACT4560.10716904506740368No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4490.10552390621768477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)