FastQCFastQC Report
Sun 21 May 2023
EGAF00007924412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449962
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA166303.6958676510460884No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA101802.2624132704539495No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA95202.1157342175561493No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT78521.745036247505345No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG18210.4047008414043852No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18200.4044786004151462No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18000.4000337806303643No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT17560.39025517710384433No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17310.38469915237286706No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC16970.37714295873873793No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16740.37203141598623884No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16640.3698090060938479No Hit
ATTCAGTCCCTGATCGAAGAAAGCGAAGAAC14400.32002702450429144No Hit
ATGTCCAACATGGACATCGATGGTATTAACA11620.2582440294958241No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG10240.22757477298082948No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10020.22268547121756951No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC9870.2193518563789831No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA9470.21046221680941948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9350.20779532493855035No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9330.20735084296007217No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9150.2033505051537685No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8820.19601655250887853No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8280.1840155390899676No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT8050.1789039963374685No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7810.1735702125957303No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7600.1689031518217094No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7110.15801334334899392No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7040.15645765642432027No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6660.1480124988332348No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6550.1455678479516048No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6210.1380116543174757No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC6210.1380116543174757No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA6190.1375671723389975No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6170.13712269036051933No Hit
AACCTGTCCTGGACCGCCAAAGCGGACAAGT5850.1300109787048684No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5370.11934341122139204No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5270.11712100132900112No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5140.11423186846889292No Hit
GCTCTGGAAGGCATCTCTAAAGACCAGGAAG5000.11112049461954566No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4920.10934256670563292No Hit
CCGAACGTTCCGGTACAGCGTGCTCGCATTT4800.10667567483476383No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4670.10378654197465563No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4610.10245309603922109No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4570.10156413208226471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)