FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426728
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA155373.640960986858139No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA94912.2241334058229127No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA88512.074154965223749No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT71621.6783524868300184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41240.9664235766108621No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17380.4072852027521044No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT17250.4042387656774339No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16860.39509945445342237No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG16270.3812733169606869No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16060.37635214937852685No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15760.369321909975441No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15720.36838454472169624No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC15560.36463508370671716No Hit
ATTCAGTCCCTGATCGAAGAAAGCGAAGAAC14100.3304212519450329No Hit
ATGTCCAACATGGACATCGATGGTATTAACA10910.25566637295888717No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9560.22403029564500104No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC9260.2170000562419152No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9230.21629703230160663No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9070.21254757128662755No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA8400.1968467032864026No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8160.19122251176393393No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8000.18747305074895484No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7770.18208320053992239No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7310.1713035001218575No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7030.16474194334564407No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7020.16450760203220788No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6880.16122682364410115No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6870.16099248233066496No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6670.15630565606194108No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA6090.14271385988264187No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC6070.1422451772557695No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6050.1417764946288971No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6050.1417764946288971No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5370.12584128531523592No Hit
AACCTGTCCTGGACCGCCAAAGCGGACAAGT5360.12560694400179973No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5160.12092011773307586No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4760.11154646519562814No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4750.11131212388219194No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4740.11107778256875575No Hit
GCTCTGGAAGGCATCTCTAAAGACCAGGAAG4720.11060909994188337No Hit
CCGAACGTTCCGGTACAGCGTGCTCGCATTT4700.11014041731501098No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4570.10709398024034045No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4350.1019384713447442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)