FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007924416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455830
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA167203.668034135532984No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA104032.282210473202729No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA95402.092885505561284No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT75831.6635587828795824No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18750.4113375600552837No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18510.40607243928657616No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT18440.4045367790623698No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG17350.3806243555711559No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17330.38018559550709696No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16930.37141039422591754No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16910.3709716341618586No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC16700.3663646534892394No Hit
ATTCAGTCCCTGATCGAAGAAAGCGAAGAAC14810.3249018274356668No Hit
ATGTCCAACATGGACATCGATGGTATTAACA11690.25645525744246755No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG10400.22815523331066404No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC10310.2261808130223987No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10160.22289011254195643No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9930.2178443718052783No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA9670.21214049097251167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9020.19788078889059518No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8840.19393194831406446No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8760.19217690805782858No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8670.19020248776956322No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7870.17265208520720446No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7770.1704582848869096No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7720.16936138472676218No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7400.16234122370181867No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7090.15554044270890466No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6980.1531272623565803No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC6750.14808152161990215No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6410.14062260053089967No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6330.1388675602746638No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5890.12921483886536647No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA5850.12833731873724855No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5240.11495513678344998No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5200.11407761665533202No Hit
CCGAACGTTCCGGTACAGCGTGCTCGCATTT5170.11341947655924359No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5160.11320009652721408No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5020.11012877607880131No Hit
AACCTGTCCTGGACCGCCAAAGCGGACAAGT5010.10990939604677182No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4810.10552179540618213No Hit
GCTCTGGAAGGCATCTCTAAAGACCAGGAAG4770.10464427527806419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)