FastQCFastQC Report
Wed 31 May 2023
EGAF00007924418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431816
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA156273.6189024955073457No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA97012.2465587194545824No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA89862.0809789354725163No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT73821.7095244270707892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38450.8904255516238397No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17390.40271782425848046No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG17160.39739148155695947No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17150.397159901439502No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT17100.3960020008522148No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16690.38650721603646No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16550.38326509439205586No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC15940.3691387072271523No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15460.35802286158919544No Hit
ATTCAGTCCCTGATCGAAGAAAGCGAAGAAC13100.3033699538692406No Hit
ATGTCCAACATGGACATCGATGGTATTAACA11650.26979083683791244No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9710.22486429405116992No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9580.2218537525242233No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9460.21907479111473405No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC9460.21907479111473405No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA8990.20819052559423457No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8650.2003168016006818No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8330.19290623784204383No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7980.1848009337310336No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7310.16928506586138542No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7240.16766400503918336No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7110.1646534635122367No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7070.16372714304240696No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6600.15284287752190748No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6250.1447375734108972No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC6090.14103229153157826No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6070.14056913129666337No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA5980.13848491023954648No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5970.13825333012208904No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5490.12713748448413212No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5310.1229690423698983No Hit
CCGAACGTTCCGGTACAGCGTGCTCGCATTT5270.12204272190006855No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5200.1204216610778665No Hit
AACCTGTCCTGGACCGCCAAAGCGGACAAGT5100.11810585990329214No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5010.11602163884617522No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4710.10907423532245215No Hit
GCTCTGGAAGGCATCTCTAAAGACCAGGAAG4450.10305315226855882No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4390.1016636715638142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)