FastQCFastQC Report
Wed 31 May 2023
EGAF00007924435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321403
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28410.8839369887648839No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14400.4480356437245452No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA11820.3677625908905642No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT11340.3528280694330793No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10130.31518062992566964No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG9810.3052242822873464No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9360.2912231684209544No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT9000.28002227732784074No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA7090.22059532736159898No Hit
CGGGCAGGACAGACCAGAGGTCAGCTTACAGCGGCATACGTTTG7050.21935078390680857No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6680.20783875694999734No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC5790.18014766508091087No Hit
ACCGTGCTGGGAGAACAGGCGACCGGCGCTTTTCGGGTTTGCCT5270.16396860016863563No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC5140.15992383394056683No Hit
TTCCTGGTTAACGCCGATCATCGGCTCTTTCTGCTGTGCGTCAA4920.1530788449392196No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC4790.14903407871115082No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4470.13907773107282756No Hit
TTCTGCGGAGTTCGGTACGATTTCCAGCTGCGGAACTTTATATT4280.13316614966257315No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG4110.12787683997971394No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4080.12694343238862113No Hit
TTCGATGGTATCCGGTTCGTCAGAGTTGCTTTCCAGTGCCAGTT4080.12694343238862113No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT3880.12072071511466911No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA3850.11978730752357633No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC3740.11636481302290272No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT3560.11076436747634591No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG3450.1073418729756723No Hit
GTCGGTGCAGTTCAGACAAGTTGGGCACAGTTTACCACGAGTTT3440.10703073711197468No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA3430.10671960124827708No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT3410.10609732952088187No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT3310.10298597088390588No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC3310.10298597088390588No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG3300.10267483502020827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)