FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411420
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA46791.1372806377910651No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT28810.7002576442564775No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19710.47907248067668073No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19230.4674055709493948No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18010.43775217539254285No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16660.4049389917845511No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15070.36629235331291626No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13700.3329930484662875No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13130.3191385931651354No Hit
CCGGCTCTGGGCGCTCGTCGTAAGAAAAAAA11290.2744154392105391No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11200.272227893636673No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9870.23990083126731807No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8210.19955276846045406No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7820.19007340430703418No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7390.17962179767634048No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG6890.16746876671041758No Hit
CCGGAAAAACTGCCGAAAACTATCGAGAAAC6660.16187837246609305No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6570.1596908268922269No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC6540.15896164503427154No Hit
TCCGAGCGTTATCTGGGTTACCTGGAACAGC6360.1545865538865393No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6070.14753779592630403No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6020.14632249282971174No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA5980.1453502503524379No Hit
GCCAAACCGGTAGCGGTTCGCTCTCCGGCAC5730.13927373486947645No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5680.13805843177288415No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5670.1378153711535657No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5660.13757231053424726No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA5340.12979437071605657No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA5170.1256623401876428No Hit
CTGGTTGCCTCTTCTGACGGTACCAGCAAAA5080.12347479461377667No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA5060.12298867337513977No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG4760.11569685479558602No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4710.11448155169899374No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4640.11278012736376453No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4470.10864809683535073No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4360.10597443002284769No Hit
GGCTGGCACGGTCCGAAAGCTCCGTACACCA4170.101356278255797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)