FastQCFastQC Report
Wed 31 May 2023
EGAF00007924454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391052
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51361.313380317707108No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA43711.1177541605719956No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT27580.705277047553778No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19650.5024907173470536No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16980.4342133526998967No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15350.39253091660444134No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA14280.3651688266522099No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13150.3362724138989188No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12380.3165819379519859No Hit
CCGGCTCTGGGCGCTCGTCGTAAGAAAAAAA11150.28512832053026194No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10960.28026963166023955No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9220.2357742704295081No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7440.1902560273314035No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7360.1882102635966572No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6590.16851978764972433No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG6510.16647402391497806No Hit
CCGGAAAAACTGCCGAAAACTATCGAGAAAC6480.1657068625144482No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6310.16135961457811238No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6080.15547804384071684No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC6040.1544551619733437No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5900.15087507543753773No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5830.14908503216963473No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5620.14371490236592577No Hit
GCCAAACCGGTAGCGGTTCGCTCTCCGGCAC5440.13911193396274665No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA5410.1383447725622168No Hit
TCCGAGCGTTATCTGGGTTACCTGGAACAGC5400.1380890520953735No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5360.13706617022800038No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA5210.1332303632253511No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA4950.12658163108742573No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA4910.12555874922005258No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4700.12018861941634361No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4680.11967717848265703No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG4550.11635281241369434No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4460.11405132821210477No Hit
CTGGTTGCCTCTTCTGACGGTACCAGCAAAA4160.10637971420680627No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC3930.10049814346941072No Hit
GTCGCGGGTAAACGTGAAGGTCTGGGCGGTC3930.10049814346941072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)