FastQCFastQC Report
Sun 21 May 2023
EGAF00007924468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412858
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33050.8005173691680917No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21300.5159158839116597No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG20310.4919366949411178No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19810.47982599344084403No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18330.44397831700003393No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT15090.3655009712782603No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13780.3337709333475432No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12930.3131827407970779No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12650.3064007479569246No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12200.2955011166066783No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12140.29404783242664545No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12130.29380561839664No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11330.2744284959962021No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11240.2722485697261528No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC10900.26401329270596674No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG10120.24512059836553973No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC9890.2395496756754138No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8530.20660856759466936No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8480.205397497444642No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8010.1940134380343847No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7980.19328679594436826No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7780.1884425153442588No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7640.18505151892418217No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7530.18238716459412194No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7390.17899616817404532No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7280.1763318138439851No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7180.17390967354393036No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7150.17318303145391392No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG6730.16301004219368403No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG6390.15477476517349792No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6280.1521104108434377No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6270.15186819681343222No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5850.1416952075532023No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5750.13927306725314756No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5690.13781978307311474No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5630.1363664988930819No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5460.1322488603829888No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5330.12910007799291767No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5030.12183365709275344No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4980.12062258694272607No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA4840.11723159052264943No Hit
TCTATCGTAGTGACCGGCAACGCAGGTTCCG4810.11650494843263301No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4800.11626273440262754No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4650.11262952395254541No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4610.11166066783252354No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4500.10899631350246332No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4360.10560531708238667No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4360.10560531708238667No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4240.102698748722321No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA4160.1007610364822772No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4150.10051882245227173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)