FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382577
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50351.316074933934868No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28880.7548807168230186No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19550.5110082414781861No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17780.46474304519090276No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17370.4540262483107975No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16990.4440936072999684No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT13410.35051767356636704No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12300.32150390640315546No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA11910.3113098801025676No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11500.3005930832224624No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11320.29588814800680646No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11030.28830797460380525No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10720.2802050306212867No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10100.2639991426562496No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC10100.2639991426562496No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9850.257464510412283No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC9260.2420427783165219No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7620.1991755907961012No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7480.19551619673947987No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7190.18793602333647869No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7090.18532217043889204No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6780.1772192264563735No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6750.1764350705870975No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6640.1735598323997522No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6640.1735598323997522No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6630.17329844710999354No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6450.1685935118943376No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6270.16388857667868167No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5880.15369455037809382No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5870.15343316508833515No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5550.14506883581605795No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5490.14350052407750596No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5270.13775004770281538No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5220.13644312125402205No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5160.1348748095154701No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5130.1340906536461941No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5020.13121541545884882No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4880.12755602140222752No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4810.12572632437391687No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4780.1249421685046409No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4710.12311247147633025No Hit
TCTATCGTAGTGACCGGCAACGCAGGTTCCG4450.11631645394260502No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA4430.11579368336308768No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4420.11553229807332903No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4330.11317983046550106No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4240.1108273628576731No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4150.10847489524984513No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC3880.10141749242636124No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC3880.10141749242636124No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA3850.10063333655708523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)