FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392650
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT21060.5363555329173564No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA20150.5131796765567299No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19720.5022284477269833No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19200.48898510123519673No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA16690.4250604864383038No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14720.37488857761365085No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14380.36622946644594423No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG13190.3359225773589711No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12250.3119826817776646No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG11890.3028142111295047No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11040.28116643321023815No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG10290.2620654526932382No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9730.24780338724054501No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG9090.23150388386603843No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT8740.22259009295810517No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8320.21189354386858525No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7710.19635807971475872No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7440.18948172672863872No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6870.17496498153571882No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6160.15688271997962563No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6130.15611868075894564No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6100.15535464153826561No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6030.15357188335667898No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5590.1423659747867057No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5210.13268814465809245No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5060.12886794855469247No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT4680.11919011842607921No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT4640.11817139946517254No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4560.11613396154335923No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4420.11256844518018592No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG4410.11231376543995925No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4400.11205908569973258No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG4190.10671081115497262No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4140.1054374124538393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)