FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007924474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403246
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44851.1122242998070657No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27170.6737822569845702No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21270.5274695843232171No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21100.5232537954499239No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18550.46001696235052547No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17010.4218268749101045No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15180.3764451476270068No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC14760.36602966923416475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12830.31816806614324755No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12630.31320831452760844No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12580.3119683766236987No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC11160.27675414015266114No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10180.2524513572360296No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10170.25220336965524764No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9730.24129191610084166No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC9490.2353402141620748No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9170.22740461157705222No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8540.21178139398778908No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7330.1817748967131726No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7280.18053495880926285No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6990.17334331896658614No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6950.17235136864345832No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6650.1649117412199997No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6650.1649117412199997No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6540.1621838778313982No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5400.1339132936222554No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5290.1311854302336539No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5140.12746561652192456No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4750.11779410087142837No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4640.11506623748282686No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4300.10663465973624041No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4270.10589069699389454No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4260.10564270941311259No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4230.10489874667076672No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4200.10415478392842087No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4120.10217088328216523No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4090.10142692053981936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)