FastQCFastQC Report
Wed 31 May 2023
EGAF00007924486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436296
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT50051.1471569759979463No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22800.5225810000550085No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT21350.4893466820690541No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14230.3261547206483672No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14080.3227166877532684No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13940.31950785705117624No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13800.31629902634908413No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13550.31056897152391955No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13540.31033976933091295No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA12570.28810715660927444No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC12120.2777930579239782No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11420.26174890441351745No Hit
GCTGACGCAAAACTGGTTTGCAACCGTTGTC10030.22988979958560243No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9790.22438894695344444No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9490.21751288116324696No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9120.20903240002200343No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT8130.18634138291435173No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7790.1785485083521279No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC7620.17465207107101602No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7380.16915121843885803No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC7290.16708839870179876No Hit
CGTGTTCGTGGCCACTATCGTGCGCCATGGG7260.16640079212277903No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT7020.16089993949062104No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG6950.15929552413957496No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6870.1574619065955223No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6820.1563158956304894No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6730.15425307589343015No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6710.153794671507417No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA6530.14966903203329848No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG6020.13797972018996277No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5970.13683370922492985No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5530.12674881273264021No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5510.12629040834662705No Hit
CGCAACTACACTCCGACCCCGCCACCGGTGT5130.11758072501237692No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5030.1152887030823111No Hit
CGTGTCCGTGGCCACTACCGTGCCCCATGGG4940.11322588334525185No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4910.1125382767662321No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG4830.11070465922217944No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4820.11047545702917284No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4650.10657901974806094No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4510.10337018904596879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)