FastQCFastQC Report
Sun 21 May 2023
EGAF00007924488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408064
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52681.2909739648682559No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46721.144918444165621No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20180.49453026976160597No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT19320.47345514429109165No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13530.3315656367628607No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13270.3251940872020075No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12550.30754979611041405No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12460.3053442597239649No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12350.30264860414052697No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10960.2685853199498118No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC10900.2671149623588457No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA10850.2658896643663739No Hit
GCTGACGCAAAACTGGTTTGCAACCGTTGTC9300.22790542659974905No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8780.21516232747804268No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8590.21050619510664995No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG7670.1879607120451694No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT7390.18109904328732748No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7000.17154171894604767No Hit
CGTGTTCGTGGCCACTATCGTGCGCCATGGG6900.16909112296110412No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6450.15806344102885822No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC6280.1538974278544542No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6160.15095671267252195No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6080.14899623588456715No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG6020.147525878293601No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC5960.1460555207026349No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA5880.14409504391468006No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5860.14360492471769135No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5730.14041914993726473No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5700.1396839711417817No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5680.13919385194479297No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG5600.13723337515683814No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5280.12939146800501883No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4870.11934402446675031No Hit
CGCAACTACACTCCGACCCCGCCACCGGTGT4710.11542307089084065No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4630.11346259410288581No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4600.11272741530740275No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4600.11272741530740275No Hit
CGTGTCCGTGGCCACTACCGTGCCCCATGGG4540.11125705771643664No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4470.10954164052697617No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG4140.10145467377666248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)