FastQCFastQC Report
Sun 21 May 2023
EGAF00007924518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539400
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT63661.1802002224694104No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA53710.9957360029662589No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28570.5296625880608083No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24880.46125324434556914No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21010.38950685947348906No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17110.3172043010752688No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14540.2695587690025955No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13080.2424916573971079No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA12360.22914349276974416No Hit
CTGATGCGTATGAAGGAAGTATCTAGCGTTT12290.2278457545420838No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11760.21802002224694103No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT11270.2089358546533185No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10340.19169447534297365No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9960.18464961067853172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9410.17445309603262885No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8860.164256581386726No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7830.14516129032258066No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG7540.13978494623655913No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7460.13830181683351872No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7010.12995921394141638No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6950.12884686688913607No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6870.12736373748609567No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6750.12513904338153503No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6740.124953652206155No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6590.1221727845754542No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6450.11957730812013348No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6370.11809417871709307No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6330.11735261401557287No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6110.11327400815721173No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6100.11308861698183167No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6090.11290322580645162No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6040.11197626992955134No Hit
GAAGACGGTAAGGTTATGGTCCTGTGCAACC5920.10975157582499073No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5730.10622914349276975No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5690.10548757879124952No Hit
CGTACCCTGAACGAACGTCTGTCTCGTGCGC5630.10437523173896922No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5410.10029662588060809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)