FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538157
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61531.1433466441949096No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA54491.0125298007830428No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT29460.5474238930274994No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24780.4604604232593834No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20180.37498350853003865No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17940.3333599674444446No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14250.26479261628112244No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12830.23840626434293338No Hit
CTGATGCGTATGAAGGAAGTATCTAGCGTTT12600.23413241860646616No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA12370.2298585728699989No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT11600.21555048062182597No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11510.21387810620320835No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10190.18934994806348332No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9780.18173135348978087No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9770.18154553410993446No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8900.1653792480632975No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG7730.14363838062126852No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7650.1421518255824973No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7450.13843543798556926No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7440.13824961860572285No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7140.1326750372103308No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7100.13193175969094523No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6900.12821537209401718No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6660.12375570697770354No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6590.12245497131877872No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6550.1217116937993931No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6510.1209684162800075No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6480.12041095814046829No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6400.11892440310169708No Hit
CGTACCCTGAACGAACGTCTGTCTCGTGCGC6200.11520801550476906No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6090.11316400232645864No Hit
GAAGACGGTAAGGTTATGGTCCTGTGCAACC6070.11279236356676584No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6010.11167744728768743No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5970.11093416976830181No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5970.11093416976830181No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5870.1090759759698378No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5510.10238647829536734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)