FastQCFastQC Report
Wed 31 May 2023
EGAF00007924521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525275
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA32460.6179620198943411No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28710.5465708438437009No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA26720.5086859264194945No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT25410.4837466089191376No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21710.41330731521583935No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG15760.300033315882157No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15430.29375089238970065No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14040.26728856313359667No Hit
CTGTGCGCGGTCGCCGCTACCCGGAGACACCAGACCGCCCGGCA11880.22616724572842797No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA11340.21588691637713578No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10450.19894341059445053No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10020.19075722240731047No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG9780.1861881871400695No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT9470.1802865165865499No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7530.14335348150968538No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA7490.14259197563181192No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7330.13954595212031792No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7250.13802294036457094No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6680.12717148160487363No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6660.1267907286659369No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6360.12107943458188566No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC6350.1208890581124173No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6330.12050830517348055No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6310.12012755223454381No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6280.11955642282613868No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6270.11936604635667031No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5960.11346437580315072No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5740.10927609347484651No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5730.10908571700537814No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5730.10908571700537814No Hit
GCGGATGATGCCCAGAATTTCGTCGCGGTGTGCCAGGCGATCAT5690.10832421112750464No Hit
TGCCAGTTCTTTACGACAACCGCCGTCGTGACGTTTGTCCACGG5630.1071819523106944No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT5400.1028032935129218No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5330.1014706582266432No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5310.10108990528770645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)