FastQCFastQC Report
Sun 21 May 2023
EGAF00007924565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485342
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27390.564344318027288No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15440.3181261873070948No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG13420.2765060514029282No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12450.2565201445578582No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA11280.2324134321777221No Hit
GGTCTCACCCGGGTAGAAAGAACCACCTTTCGGCGGTACAACCA10040.20686443786031294No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA9340.19244161848758196No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT9080.18708457129199615No Hit
CAGCTGCTGCAGCGGGGTAGTTTCGCCCGGATAGATAGAACCAC9050.18646645046173627No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8610.17740067828459108No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8150.16792282555393928No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG8140.16771678527718598No Hit
CAGGGCTGGGATGCCCCAGAAAATCATCTGCTGCAGCTGCTGAG7880.16235973808160017No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7870.1621536978048469No Hit
GGACACCTGCTGTGGAGAAGTAACGCTCGGGTAATAACGTTTCA7870.1621536978048469No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7850.16174161725134029No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7650.1576208117162743No Hit
GGTTTCGCCCGGGTAGAAGCTACCACCTTTTGGCGGAACAACGG7270.1497912811996489No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT6830.14072550902250372No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6640.13681074376419103No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6500.13392617988964484No Hit
CAGTTGCTGCAGCGGAGTGGTTTCACCCGGGTAGATGGAACCGC6230.12836309241730573No Hit
GGTCTCGCCCGGGTAGAAAGAGCCACCTTTCGGTGGAACTACGG6000.12362416605197984No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5990.12341812577522655No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5930.12218188411470675No Hit
GGCACCAACGAACGCACGCACAGCGGATGCGGTTACGTCCTGCG5920.12197584383795346No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5880.12115168273094024No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5770.11888523968665395No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5700.11744295774938085No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5600.11538255498184785No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5550.11435235359808135No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT5340.11002550778626205No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5290.10899530640249555No Hit
TACTTGCTGCGGGCTGGTAACGGATGGGTAGTAGCGTTTCAGCA4980.10260805782314328No Hit
CAGCGCGCGCAGGCCCTCAGCGATGTTTTCGAACTTCGGGTTGG4930.10157785643937678No Hit
TTGCTGCAGCTGTTGCGGCGGGGTGGTTTCGCCCGGGTAGAAGG4900.10095973560911688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)