FastQCFastQC Report
Sun 21 May 2023
EGAF00007924568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480337
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT53331.110262170101408No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38060.7923603636613461No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG35960.7486410582570154No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22290.4640491987916817No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21100.43927492572922755No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18560.38639538490684666No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17860.3718222831054031No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17580.36599304238482566No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC17320.36058017600143233No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15300.31852636794583805No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14990.31207256571948444No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12590.26210764525739216No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12380.2577357147169591No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12170.2533637841765261No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12100.25190647399638166No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11900.2477427306245407No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11410.2375415593635302No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11280.23483512617183352No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG10910.22713220093392764No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10680.22234389605631047No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10580.22026202437038997No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10480.21818015268446944No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10450.21755559117869328No Hit
CGTTCTGGCTACTCCTCCCCAGGCAGCCCGG10410.21672284250432508No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10100.2102690402779715No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10080.20985266594078741No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9800.20402342522020997No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9740.20277430220865766No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9350.1946550026335677No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9200.19153219510468691No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA8910.18549476721551744No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8890.18507839287833333No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8600.17904096498916386No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA8130.16925616806533747No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8050.16759067071660105No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7930.16509242469349644No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC7210.15010294855486878No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG7050.14677195385739594No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6970.14510645650865955No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6880.14323277199133108No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC6860.14281639765414697No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6620.13781990560793775No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6500.13532165958483316No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6430.13386434940468878No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6380.13282341356172853No Hit
GGTGGCGTTCACGTTAAGCTGCCGAAACTGA6360.13240703922454444No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6220.12949241886425572No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6150.12803510868411136No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5990.12470411398663855No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5820.12116493212057369No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5440.11325381971407575No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5410.11262925820829958No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5130.10680001748772217No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5100.10617545598194601No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4950.10305264845306525No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4910.10221989977869704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)