FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478036
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52021.0882025621501308No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38290.8009856998217707No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG35990.7528721686232835No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22920.47946179785622844No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20840.43595043051150956No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18620.3895104134416655No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17910.3746579755499586No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17870.37382121848563704No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC17030.35624932013488525No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15830.3311466082052398No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14500.3033244358165494No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12310.2575119865449464No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12290.25709360801278563No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12120.2535373904894192No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11320.2368022492029889No Hit
CGTTCTGGCTACTCCTCCCCAGGCAGCCCGG11140.23303684241354206No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11130.2328276531474617No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11100.23220008534922057No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11000.23010819268841678No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10620.22215900057736238No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG10600.22174062204520165No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10460.21881197232007632No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10340.21630170112711178No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10280.21504656553062948No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10230.2140006192002276No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9920.20751575195173586No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9780.20458710222661056No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9450.19768385644595804No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9440.19747466717987766No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9430.19726547791379728No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8830.18471412194897455No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8800.1840865541507334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8580.17948439029696509No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA8470.17718330837008092No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA8170.17090763038766954No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7850.16421357387309743No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC7790.16295843827661516No Hit
GGTGGCGTTCACGTTAAGCTGCCGAAACTGA7210.15082546084395318No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC7130.14915194671531015No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6860.14350383653113993No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6810.142457890200738No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6800.14224870093465763No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6680.1397384297416931No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6520.13639140148440704No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6370.13325356249320133No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6300.1317892376306387No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6280.13137085909847793No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6000.12551355964822733No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5960.12467680258390582No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5620.11756436753717293No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5490.11484490707812801No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5470.11442652854596724No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5450.11400815001380649No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5190.10856922909571665No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4940.10333949744370716No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4930.10313030817762679No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4920.10292111891154641No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4900.10250274037938566No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4800.10041084771858187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)