FastQCFastQC Report
Sun 21 May 2023
EGAF00007924572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464128
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56121.2091492002206288No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38130.821540609487038No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC36280.781680915609487No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG33340.7183363210148924No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21330.4595714975179261No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20330.43802571704357424No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18680.4024751792608935No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17600.3792057363485935No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17300.37274200220628795No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15210.3277113210148925No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15060.32447945394373967No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13900.2994863485934915No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11980.2581184500827358No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11620.2503619691119691No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11480.24734555984555984No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11440.2464837286265858No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11290.243251861555433No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11200.2413127413127413No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11140.2400199944842802No Hit
CGTTCTGGCTACTCCTCCCCAGGCAGCCCGG10710.2307553088803089No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG10470.2255843215664644No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10030.21610417815774957No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9830.2117950220628792No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9760.2102868174296746No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9690.20877861279646995No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9680.20856315499172642No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9600.20683949255377826No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9590.20662403474903476No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9270.19972938499724213No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA9040.1947738554881412No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8940.192619277440706No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8770.18895649476006618No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8320.17926089354660782No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7920.17064258135686708No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7470.16094698014340872No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC7290.15706873965802537No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA7230.15577599282956425No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC6830.14715768063982348No Hit
GGTGGCGTTCACGTTAAGCTGCCGAAACTGA6500.14004757308328736No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6340.13660024820739106No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6290.13552295918367346No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6210.1337992967457253No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6170.13293746552675126No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6150.1325065499172642No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6100.13142926089354662No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5990.1290592250413679No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5910.12733556260341974No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5830.12561190016547158No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5620.1210872862658577No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5470.11785541919470491No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5340.11505446773303915No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5160.11117622724765581No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5130.11052985383342526No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4910.10578978212906784No Hit
AGCCAGCCGGATGCAGTACTGGCTGCGACTT4690.10104971042471042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)