FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483582
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT109842.2713831366758894No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG53821.1129446505453058No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41260.8532162073857175No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG29040.6005186297256722No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26490.5477871384790998No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19290.3988982220181893No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC19260.3982778515329355No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC17310.3579537699914389No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16090.33272537025778465No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC16080.33251858009603336No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC15800.3267284555669979No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC14310.2959167214660595No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC12550.25952165299783697No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT12340.2551790596010604No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC12170.2516636268512889No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11390.2355339942346903No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11200.23160498116141628No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11110.22974386970565489No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA10130.20947843385403098No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9890.20451546997200062No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9590.1983117651194627No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9020.1865247258996406No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8940.18487040460563048No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA7390.1528179295341845No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7140.14764817549040285No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6930.14330558209362632No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6300.13027780190329666No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6260.12945064125629158No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6040.12490125769776377No Hit
GCACCGAGCGTTGATCCAATCGTGGAGGCTA5960.12324693640375366No Hit
GCGGAGCAGCACACGCCGATGAAGGCGCACG5890.12179940527149481No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5880.12159261510974353No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5820.12035187413923595No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5640.1166296512277132No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5580.1153889102572056No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5500.11373458896319548No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5410.1118734775074341No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC5370.11104631686042905No Hit
GCGCTGAAGGCGGGCGCAGTTGAAGCGGCGT5290.10939199556641893No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG5240.10835804475766261No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5220.10794446443416009No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5200.10753088411065756No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5140.10629014314014996No Hit
GCTAAACTGAACCCGTCTAGCATCAAGGCCC4930.10194754974337342No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4880.1009135989346171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)