FastQCFastQC Report
Sun 21 May 2023
EGAF00007924577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503503
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA57211.1362395060208181No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT56781.127699338434925No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA50020.993439959642743No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT28160.5592816726017521No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA20460.4063530902497105No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG20280.4027781363765459No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG17150.34061366069318355No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG15820.3141987237414673No Hit
CGGGATTTCTTCGTAGTCGTTTTCATACCAATCCGGGTTGTGCA15800.31380150664444895No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG15280.30347386212197347No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC14130.2806338790434218No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13770.27348397129709257No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12810.25441755064021465No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12700.25223285660661404No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG12660.2514384224125775No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12560.24945233692748606No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT12550.2492537283789769No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11670.23177617611017215No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG11560.22959148207657154No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT10860.21568888368093142No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT10320.2049640220614376No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG9510.18887672963219682No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9170.1821240389828859No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8580.17040613462084636No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA8140.16166735848644398No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7880.1565035362252062No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7460.14816197718782212No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6840.13584824718025512No Hit
AGATGGCGCGTTGGAAGTAACTTTGTTGTTGGTGTCATCGGCGG6820.13545103008323686No Hit
TTTTACGTCTTCCGGAATTGCTTTGCGACGGCGTTTTTTACCTT6500.1290955565309442No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6470.12849973088541677No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT6460.12830112233690763No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6330.12571921120628876No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6220.12353451717268815No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA6100.12115121459057841No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT5950.11817208636294124No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA5840.11598739232934065No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5830.11578878378083149No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG5730.11380269829574005No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5630.1118166128106486No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5480.10883748458301143No Hit
AGACAGGCTTTTACGAGTGGACGCAACCTGTTTACCGACAGCGT5410.10744722474344741No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5400.10724861619493826No Hit
CAGCAGAGTACCACCTTTAGCGTCCATAATGATATCACCCTGGG5400.10724861619493826No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5150.10228340248220964No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG5090.10109175119115477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)