FastQCFastQC Report
Sun 21 May 2023
EGAF00007924578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503503
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT112552.235339213470426No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG56961.1312742923080896No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG29910.5940381685908525No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA28190.5598774982472796No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC21540.42780281348869814No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC20710.4113183039624392No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC18660.37060355151806446No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC17940.356303736025406No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC17200.34160670343572924No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC16560.328895756331144No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC15570.30923351002873867No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC13690.2718951029090194No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC13140.2609716327410164No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12810.25441755064021465No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT12560.24945233692748606No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12540.24905511983046774No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12270.2436926890207208No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA10820.21489444948689482No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10310.20476541351292843No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10080.2001974168972181No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9770.19404055189343458No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9660.191855857859834No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA8490.16861865768426404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8480.16842004913575492No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7580.15054527976993184No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7410.1471689344452764No Hit
GCGGAGCAGCACACGCCGATGAAGGCGCACG7030.13962180960192888No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6660.13227329330709053No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6640.13187607621007225No Hit
GCACCGAGCGTTGATCCAATCGTGGAGGCTA6610.1312802505645448No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6590.1308830334675265No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6580.13068442491901736No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6510.12929416507945335No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6180.12274008297865158No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC5990.11896652055697782No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5910.11737765216890467No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5730.11380269829574005No Hit
GCTAAACTGAACCCGTCTAGCATCAAGGCCC5610.1114193957136303No Hit
GCGCTGAAGGCGGGCGCAGTTGAAGCGGCGT5550.11022774442257544No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG5530.10983052732555715No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA5500.1092347016800297No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5490.10903609313152056No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5480.10883748458301143No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5480.10883748458301143No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5410.10744722474344741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)