FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487720
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT55951.1471746083818584No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA55181.1313868613138687No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA48931.003239563684081No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT27780.5695891085048799No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA20010.41027638809152794No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG19790.4057656032149594No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG16060.3292872959895022No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG15720.3223160829984417No Hit
CGGGATTTCTTCGTAGTCGTTTTCATACCAATCCGGGTTGTGCA14710.30160747970146806No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG14070.2884851964241778No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13300.272697449356188No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG12950.2655212006889199No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC12840.2632658082506356No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC12830.26306077257442795No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12120.2485032395636841No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11730.24050684819158533No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11110.22779463626671043No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT10940.2243090297711802No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG10830.22205363733289593No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT10740.220208316247027No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT9860.20216517674075288No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG8670.17776593127204135No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8410.17243500369064216No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8200.1681292544902813No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7840.16074797014680556No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA7590.15562207824161403No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7160.14680554416468464No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT6550.1342983679160174No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6530.13388829656360207No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6510.13347822521118674No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6510.13347822521118674No Hit
AGATGGCGCGTTGGAAGTAACTTTGTTGTTGGTGTCATCGGCGG6490.13306815385877144No Hit
TTTTACGTCTTCCGGAATTGCTTTGCGACGGCGTTTTTTACCTT6080.12466169113425736No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5780.11851062084802756No Hit
CAGCAGAGTACCACCTTTAGCGTCCATAATGATATCACCCTGGG5780.11851062084802756No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5740.11769047814319691No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5650.11584515705732798No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5590.114614943000082No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5510.11297465759042075No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT5470.1121545148855901No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG5180.10620848027556795No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG5170.10600344459936029No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5040.1033379808086607No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT4910.10067251701796112No Hit
AGACAGGCTTTTACGAGTGGACGCAACCTGTTTACCGACAGCGT4910.10067251701796112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)